{"id":136,"date":"2021-06-21T17:06:38","date_gmt":"2021-06-21T09:06:38","guid":{"rendered":"http:\/\/nnwilking.gotoip3.com\/?p=136"},"modified":"2021-06-24T16:55:05","modified_gmt":"2021-06-24T08:55:05","slug":"%e5%a4%9a%e7%bb%84%e5%ad%a6%e7%a0%94%e7%a9%b6","status":"publish","type":"post","link":"http:\/\/nnwilking.com\/x_omics\/136\/","title":{"rendered":"\u591a\u7ec4\u5b66\u7814\u7a76"},"content":{"rendered":"\n

\u8f6c\u5f55\u7ec4\u5b66\u7814\u7a76\uff08RNA-seq\uff09<\/strong><\/p>\n\n\n\n

\u8f6c\u5f55\u7ec4\u5b66\u7814\u7a76\u5305\u62ecmRNA\u3001lncRNA\u3001circRNA\u3001\u5c0fRNA\u6d4b\u5e8f\uff0c\u5dee\u5f02\u5206\u6790\u3002<\/p>\n\n\n\n

LongRNA-seq\u662f\u4e00\u79cd\u5168\u9762\u68c0\u6d4b\u7ec6\u80de\u548c\u7ec4\u7ec7\u957f\u7247\u6bb5RNA\uff08200nt\uff09 \u7684\u8f6c\u5f55\u7ec4\u6d4b\u5e8f\u65b9\u6cd5\uff0c\u5305\u62ec\u7ebf\u6027\u7684mRNA\u548clncRNA\u5206\u5b50\uff0c\u4ee5\u53ca\u73af\u72b6RNA\uff0c\u53ef\u51c6\u786e\u9ad8\u6548\u63ed\u793a\u8f6c\u5f55\u7ec4\u7684\u8868\u8fbe\u5168\u8c8c\uff0c\u662f\u975e\u7f16\u7801RNA\u7684\u91cd\u8981\u7814\u7a76\u6280\u672f\u3002<\/p>\n\n\n\n

lncRNA\u662f\u4e00\u7c7b\u957f\u5ea6\u5927\u4e8e200nt\uff0c\u65e0\u5178\u578b\u5f00\u653e\u9605\u8bfb\u6846(ORF)\u7684RNA\uff0c\u5176\u6570\u91cf\u8fdc\u9ad8\u4e8e\u7f16\u7801\u86cb\u767d\u8d28\u7684mRNA\u3002\u5b83\u4eec\u7684\u57fa\u56e0\u80cc\u666f\u5f88\u4e30\u5bcc\uff0c\u53ef\u4ee5\u662f\u7531\u542f\u52a8\u5b50\u3001\u589e\u5f3a\u5b50\u8d77\u59cb\u7684\u5e8f\u5217\uff0c\u57fa\u56e0\u5185\u542b\u5b50\uff0cRNA\u7684\u53cd\u4e49\u94fe\uff0c\u4ea6\u6216\u662f\u57fa\u56e0\u4e4b\u95f4\u7684\u5e8f\u5217\u7b49\u3002\u7814\u7a76\u53d1\u73b0lncRNA\u53ef\u5728\u8868\u89c2\u9057\u4f20\u5b66\u6c34\u5e73(\u5305\u62ecDNA \u7532\u57fa\u5316\u3001\u7ec4\u86cb\u767d\u4fee\u9970\u3001\u67d3\u8272\u8d28\u91cd\u6784)\u3001\u8f6c\u5f55\u53ca\u8f6c\u5f55\u540e\u6c34\u5e73\u8c03\u8282\u57fa\u56e0\u7684\u8868\u8fbe\uff0c\u4ece\u800c\u53c2\u4e0e\u5404\u79cd\u57fa\u672c\u751f\u547d\u6d3b\u52a8\u548c\u75be\u75c5\u7684\u53d1\u751f\u53d1\u5c55\u8fc7\u7a0b\u3002<\/p>\n\n\n\n

\u4e0e\u4f20\u7edf\u7684\u7ebf\u6027RNA\uff08linear RNA\uff0c\u542b5\u2019\u548c3\u2019\u672b\u7aef\uff09\u4e0d\u540c\uff0ccircRNA\u5206\u5b50\u5448\u5c01\u95ed\u73af\u72b6\u7ed3\u6784\uff0c\u4e0d\u53d7RNA\u5916\u5207\u9176\u5f71\u54cd\uff0c\u8868\u8fbe\u66f4\u7a33\u5b9a\uff0c\u4e0d\u6613\u964d\u89e3\u3002\u5728\u529f\u80fd\u4e0a\uff0c\u8fd1\u5e74\u7684\u7814\u7a76\u8868\u660e\uff0ccircRNA\u5206\u5b50\u5bcc\u542bmiRNA\u7ed3\u5408\u4f4d\u70b9\uff0c\u5728\u7ec6\u80de\u4e2d\u8d77\u5230miRNA\u6d77\u7ef5\uff08 miRNA sponge\uff09\u7684\u4f5c\u7528\uff0c\u8fdb\u800c\u89e3\u9664miRNA\u5bf9\u5176\u9776\u57fa\u56e0\u7684\u6291\u5236\u4f5c\u7528\uff0c\u5347\u9ad8\u9776\u57fa\u56e0\u7684\u8868\u8fbe\u6c34\u5e73\uff1b\u8fd9\u4e00\u4f5c\u7528\u673a\u5236\u88ab\u79f0\u4e3a\u7ade\u4e89\u6027\u5185\u6e90RNA\uff08ceRNA\uff09\u673a\u5236\u3002\u901a\u8fc7\u4e0e\u75be\u75c5\u5173\u8054\u7684miRNA\u76f8\u4e92\u4f5c\u7528\uff0c circRNA\u5728\u75be\u75c5\u4e2d\u53d1\u6325\u7740\u91cd\u8981\u7684\u8c03\u63a7\u4f5c\u7528\u3002\u800c\u6700\u65b0\u7684\u7814\u7a76\u53d1\u73b0\uff0c\u8fd8\u5b58\u5728\u5927\u91cf\u7684\u73af\u5f62RNA\u53ef\u4f5c\u4e3a\u4fe1\u4f7fRNA\u6765\u7f16\u7801\u86cb\u767d\uff0c\u8fd9\u4e9b\u73af\u5f62\u4fe1\u4f7fRNA\u901a\u8fc7\u4e00\u79cd\u5e38\u89c1\u7684RNA\u7532\u57fa\u5316\u4fee\u9970m6A\uff0c\u6765\u9a71\u52a8\u975e\u5e3d\u4f9d\u8d56\u6027\u7684\u7ffb\u8bd1\u673a\u5236\u6765\u5408\u6210\u86cb\u767d\u8d28\u3002<\/p>\n\n\n\n

RNA m6A\u68c0\u6d4b<\/strong><\/p>\n\n\n\n

\u771f\u6838\u57fa\u56e0\u901a\u5e38\u4ea7\u751f\u591a\u79cdRNA\u5f02\u6784\u4f53\uff08isoform\uff09\uff0c\u53ef\u4ee5\u4ea7\u751f\u529f\u80fd\u4e0a\u4e0d\u540c\u7684\u86cb\u767d\u8d28\u53d8\u4f53\u3002\u4f20\u7edf\u4e8c\u4ee3\u6d4b\u5e8f\u5b9e\u9645\u4e0a\u662f\u4e00\u79cd\u77ed\u8bfb\u957fRNA-seq\uff0c\u96be\u4ee5\u83b7\u5f97\u5b8c\u6574\u7684\u8f6c\u5f55\u672c\uff0c\u65e0\u6cd5\u4f53\u73b0\u5f02\u6784\u4f53\u7684\u591a\u6837\u6027\u3002<\/p>\n\n\n\n

\u57fa\u4e8eOxford Nanopore\u516c\u53f8\u7684\u7eb3\u7c73\u5b54\u6d4b\u5e8f\u5e73\u53f0\uff0c\u76f4\u63a5\u5bf9RNA\u8fdb\u884c\u6d4b\u5e8f\uff0c\u5177\u6709\u957f\u8bfb\u957f\u7684\u4f18\u52bf\uff0c\u63d0\u9ad8\u5bf9\u5f02\u6784\u4f53\u7684\u68c0\u6d4b\uff0c\u8fd8\u80fd\u76f4\u63a5\u68c0\u6d4bm6A\u3001m5C\u7b49\u6838\u9178\u4fee\u9970\uff0c\u5728\u53ef\u53d8\u526a\u5207\u7814\u7a76\u65b9\u9762\u5177\u6709\u72ec\u7279\u7684\u4f18\u52bf\u3002\u4e00\u6b21\u6d4b\u5e8f\uff0c\u5373\u53ef\u6ee1\u8db3\u591a\u9879\u8868\u89c2\u9057\u4f20\u8f6c\u5f55\u7ec4\u7814\u7a76\u9700\u6c42\uff0c\u5927\u5927\u63d0\u5347\u7814\u7a76\u7684\u9ad8\u5ea6\u3001\u6df1\u5ea6\u3001\u51c6\u786e\u6027\u3001\u6548\u7387\uff0c\u5c24\u5176\u9002\u7528\u4e8e\u591a\u7ec4\u5b66\u5206\u5b50\u673a\u5236\u53ca\u73cd\u8d35\u7684\u4e34\u5e8a\u6837\u672c\u7814\u7a76\u3002<\/p>\n\n\n\n

ONT\u4f18\u52bf\uff1a
1. \u4f5c\u4e3a\u957f\u8bfb\u957f\u6d4b\u5e8f\u6280\u672f\uff0c\u80fd\u51cf\u5c11\u77ed\u8bfb\u957f\u6d4b\u5e8f\u6253\u65ad\u540e\u91cd\u65b0\u62fc\u63a5\u5e26\u6765\u7684\u6b67\u4e49\uff0c\u7ed3\u679c\u51c6\u786e\u5ea6\u66f4\u9ad8\u3001\u66f4\u53ef\u4fe1\uff1b
2. \u80fd\u591f\u76f4\u63a5\u5bf9RNA\u8fdb\u884c\u6d4b\u5e8f\uff0c\u907f\u514d\u9006\u8f6c\u5f55\u548cPCR\u6269\u589e\u5bfc\u81f4\u7684RNA\u4fee\u9970\u4fe1\u606f\u7f3a\u5931\uff1b
3. \u53ef\u4ee5\u76f4\u63a5\u68c0\u6d4bm6A\u7b49\u78b1\u57fa\u4fee\u9970\uff0c\u914d\u5408\u4f18\u5316\u7684\u7b97\u6cd5\uff0c\u83b7\u5f97\u65e0\u635f\u7684m6A\u5355\u78b1\u57fa\u5206\u8fa8\u7387\u56fe\u8c31\uff1b
4. \u4e00\u6b21\u6d4b\u5e8f\uff0c\u591a\u5957\u6570\u636e\uff1a\u8f6c\u5f55\u7ec4\u56fe\u8c31\u3001m6A\u4fee\u9970\u56fe\u8c31\u3001\u5f02\u6784\u4f53\u3001\u53ef\u53d8\u526a\u5207\u60c5\u51b5\uff0c\u8282\u7701\u73cd\u8d35\u7684\u4e34\u5e8a\u6837\u672c\uff0c\u8282\u7701\u6837\u672c\u51c6\u5907\u65f6\u95f4\u548c\u7cbe\u529b\uff0c\u4e14\u4fdd\u6301\u4e86\u6837\u672c\u7684\u7edf\u4e00\u6027\u3002<\/p>\n\n\n\n

\u86cb\u767d\u8d28\u7ec4\u5b66<\/strong><\/p>\n\n\n\n

iTRAQ\u548cTMT\u662f\u5206\u522b\u7531\u7f8e\u56fdAB Sciex\u516c\u53f8\u548cThermo\u516c\u53f8\u7814\u53d1\u7684\u591a\u80bd\u4f53\u5916\u6807\u8bb0\u5b9a\u91cf\u6280\u672f\uff0c\u91c7\u75288\u79cd\u621610\u79cd\u540c\u4f4d\u7d20\u6807\u7b7e,\u901a\u8fc7\u7279\u5f02\u6027\u6807\u8bb0\u591a\u80bd\u7684\u6c28\u57fa\u9178\u57fa\u56e2\u540c\uff0c\u4e00\u6b21\u4e0a\u673a\u53ef\u5b9e\u73b08\u79cd\u621610\u79cd\u4e0d\u540c\u6837\u672c\u4e2d\u86cb\u767d\u8d28\u7684\u76f8\u5bf9\u5b9a\u91cf,\u662f\u8fd1\u5e74\u6765\u5b9a\u91cf\u86cb\u767d\u8d28\u7ec4\u5b66\u5e38\u7528\u7684\u9ad8\u901a\u91cf\u7b5b\u9009\u6280\u672f\u3002<\/p>\n\n\n\n

iTRAQ\u548cTMT\u539f\u7406\u7c7b\u4f3c\uff0c\u4ee5iTRAQ\u8bd5\u5242\u4e3a\u4f8b\uff0c\u8be5\u8bd5\u5242\u7ed3\u6784\u7531\u62a5\u544a\u57fa\u56e2\u3001\u4e2d\u6027\u5e73\u8861\u57fa\u56e2\u548c\u53cd\u5e94\u57fa\u56e2\u4e09\u90e8\u5206\u7ec4\u6210\u3002\u4e0d\u540c\u62a5\u544a\u57fa\u56e2\u53ca\u5176\u76f8\u5bf9\u5e94\u5e73\u8861\u57fa\u56e2\u7684\u8d28\u91cf\u548c\u90fd\u76f8\u540c\uff0c\u800c\u53cd\u5e94\u57fa\u56e2\u80fd\u4e0e\u8d56\u6c28\u9178\u03b5\u6c28\u57fa\u548c\u6240\u6709\u80bd\u94fe\u7684\u6c28\u57fa\u672b\u7aef\u8fde\u63a5\uff0c\u53ef\u6807\u8bb0\u6240\u6709\u6c28\u57fa\u9178\u3002
\u4e0d\u540c\u6807\u8bb0\u8bd5\u5242\u4e0e\u6765\u6e90\u4e8e\u4e0d\u540c\u6837\u54c1\u80f0\u9176\u6d88\u5316\u540e\u7684\u80bd\u6bb5\u7ed3\u5408\uff0c,\u7ecf\u8fc7\u8272\u8c31\u5206\u79bb\uff0c\u5e76\u901a\u8fc7\u4e00\u7ea7\u8d28\u8c31\u548c\u4e8c\u7ea7\u8d28\u8c31\u3002\u5e73\u8861\u57fa\u56e2\u5728\u4e8c\u7ea7\u8d28\u8c31\u65f6\u53d1\u751f\u4e2d\u6027\u4e22\u5931\uff0c\u800c\u62a5\u544a\u57fa\u56e2\u5728\u4e8c\u7ea7\u8d28\u8c31\u4f4e\u8d28\u91cf\u533a\u57df\u4ea7\u751f\u591a\u4e2a\u62a5\u544a\u79bb\u5b50\uff0c\u5176\u4fe1\u53f7\u5f3a\u5ea6\u5206\u522b\u4ee3\u8868\u8be5\u6807\u8bb0\u6837\u54c1\u7684\u8868\u8fbe\u91cf\uff0c\u6839\u636e\u62a5\u544a\u79bb\u5b50\u7684\u5cf0\u9762\u79ef\u8ba1\u7b97\u540c\u4e00\u86cb\u767d\u8d28\u540c\u4e00\u80bd\u6bb5\u5728\u4e0d\u540c\u6837\u54c1\u95f4\u7684\u6bd4\u503c,\u4ece\u800c\u5b9e\u73b0\u86cb\u767d\u7684\u76f8\u5bf9\u5b9a\u91cf\u3002 <\/p>\n","protected":false},"excerpt":{"rendered":"

\u8f6c\u5f55\u7ec4\u5b66\u7814\u7a76\uff08RNA-seq\uff09 \u8f6c\u5f55\u7ec4\u5b66\u7814\u7a76\u5305\u62ecmRNA\u3001lncRNA\u3001circRNA\u3001\u5c0fRNA\u6d4b\u5e8f […]<\/p>\n","protected":false},"author":2,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":[],"categories":[6],"tags":[],"_links":{"self":[{"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/posts\/136"}],"collection":[{"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/comments?post=136"}],"version-history":[{"count":3,"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/posts\/136\/revisions"}],"predecessor-version":[{"id":227,"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/posts\/136\/revisions\/227"}],"wp:attachment":[{"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/media?parent=136"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/categories?post=136"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/nnwilking.com\/wp-json\/wp\/v2\/tags?post=136"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}