{"id":121,"date":"2021-06-21T15:54:56","date_gmt":"2021-06-21T07:54:56","guid":{"rendered":"http:\/\/nnwilking.gotoip3.com\/?p=121"},"modified":"2021-06-21T17:55:08","modified_gmt":"2021-06-21T09:55:08","slug":"%e8%9b%8b%e7%99%bd%e8%b4%a8%e6%a3%80%e6%b5%8b","status":"publish","type":"post","link":"http:\/\/nnwilking.com\/bio_tech\/121\/","title":{"rendered":"\u86cb\u767d\u8d28\u68c0\u6d4b"},"content":{"rendered":"\n<p><strong>Western Blot\u68c0\u6d4b\uff08\u86cb\u767d\u5370\u8ff9\u6742\u4ea4\uff09<\/strong><\/p>\n\n\n\n<p>Western Blot\u91c7\u7528\u7684\u662f\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\uff0c\u88ab\u68c0\u6d4b\u7269\u662f\u86cb\u767d\u8d28\uff0c\u201c\u63a2\u9488\u201d\u662f\u6297\u4f53\uff0c\u201c\u663e\u8272\u201d\u7528\u6807\u8bb0\u7684\u4e8c\u6297\u3002\u7ecf\u8fc7\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3(PAGE)\u5206\u79bb\u7684\u86cb\u767d\u8d28\u6837\u54c1\uff0c\u901a\u8fc7\u8f6c\u819c\u65b9\u5f0f\u8f6c\u79fb\u5230\u56fa\u76f8\u8f7d\u4f53(\u5982PVDF\u3001NC\u819c)\u4e0a\uff0c\u56fa\u76f8\u8f7d\u4f53\u4ee5\u975e\u5171\u4ef7\u952e\u5f62\u5f0f\u5438\u9644\u86cb\u767d\u8d28\u3002\u4ee5\u56fa\u76f8\u8f7d\u4f53\u4e0a\u7684\u86cb\u767d\u8d28\u6216\u591a\u80bd\u4f5c\u4e3a\u6297\u539f\uff0c\u4e0e\u5bf9\u5e94\u7684\u6297\u4f53\u8d77\u514d\u75ab\u53cd\u5e94\uff0c\u518d\u4e0e\u9176\u6807\u8bb0(\u901a\u5e38\u662fHRP)\u7684\u7b2c\u4e8c\u6297\u4f53\u8d77\u53cd\u5e94\uff0c\u7ecf\u8fc7\u5e95\u7269\u5316\u5b66\u53d1\u5149\u8bd5\u5242(\u5982ECL)\u53d1\u5149\u4ee5\u68c0\u6d4b\u7535\u6cf3\u5206\u79bb\u7684\u7279\u5f02\u6027\u76ee\u7684\u57fa\u56e0\u8868\u8fbe\u7684\u86cb\u767d\u6210\u5206\u3002<\/p>\n\n\n\n<p><\/p>\n\n\n\n<p><strong>ELISA( 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Blot\u68c0\u6d4b\uff08\u86cb\u767d\u5370\u8ff9\u6742\u4ea4\uff09 Western Blot\u91c7\u7528\u7684\u662f\u805a\u4e19\u70ef\u9170\u80fa\u51dd\u80f6\u7535\u6cf3\uff0c\u88ab 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